Analysis of mRNA expression using oligonucleotide microarrays
نویسندگان
چکیده
Fly stocks Fly culture and crosses were grown on standard fly medium at 25 8C. Oregon R were used as wild-type standard. The BG00650 line was obtained from the Bloomington stock centre. Dredd , Relish, Dif 1 and imd were described elsewhere. The P-element inserted in BG00650 was mobilized by following standard crosses. Mobilization events were screened by PCR using different primer combinations either in the genomic DNA flanking the P-element (5 0 -GACTGCCTGAAAATCGGACTC-3 0 and 5 0 -AGCACGCTT TTGAGATAACACC-3 0 ) or inside the P-element sequence (5 0 -CTTGCCGACGGGA CCACC-3 0 ). D5 resulted from the imprecise excision of the P-element and deletes at least the first exon of PGRP-LC. N18 contains a P-element insertion in the first intron of PGRP-LC.
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